Fig. 2
From: Methylthioadenosine promotes remyelination by inducing oligodendrocyte differentiation
Methylthioadenosine promotes remyelination in mouse cerebellar cultures. a Changes in the g-ratio in organotypic cerebellar cultures treated with methylthioadenosine (MTA, 192μM, white) or the placebo (black) at different time points after lysolecithin demyelination. b The scatter plot displays the g-ratios of individual axons in function of the respective axon diameter. c Changes in the g-ratio clustering in samples relative to the respective axonal diameter. Electron microscopy images are shown from mouse cerebellar organotypic cultures showing normal myelinated axons in control tissue (d), nude axons in lysolecithin cultures not treated or treated with placebo (e, g) while remyelinated axons are shown in MTA-treated slices (f). Zoom images of myelinated, demyelinated and MTA treated remyelinated axons (h). Results are the mean of three independent experiments with six slices per condition. Between four and seven animals were used in each experiment being the slices per condition randomly selected from different animals. *demyelinated axons. # remyelinated axons.*P < 0.05, **P < 0.01. Scale bars: 1–2 μm