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Fig. 4 | Multiple Sclerosis and Demyelinating Disorders

Fig. 4

From: Methylthioadenosine promotes remyelination by inducing oligodendrocyte differentiation

Fig. 4

Methylthioadenosine promotes remyelination in the mouse cuprizone model. a Preventive trial. Top: representative images of MBP immunostaining in the corpus callosum of mice fed with a normal diet (control) or with a cuprizone supplement for 4, 5 or 6 weeks and injected intraperitoneally daily with methylthioadenosine (MTA, 60mg/kg/day) or the vehicle alone (saline) for 6 weeks (treatments started the same day as cuprizone supplementation). Bottom: quantification of the area of MBP staining shown as the mean ± standard error of the mean (n = 6 per group). b Curative trial. Top: representative images of MBP immunostaining in the corpus callosum of mice fed with normal diet (control) or with a cuprizone supplement for 5 weeks, after which the animals received a normal diet and a daily intraperitoneal injection of the vehicle alone, MTA (60mg/kg/day) or a placebo for 7, 8 or 9 weeks. Each treatment started after the cessation of cuprizone supplementation at week 5. Bottom: quantification of the area stained for MBP. The values shown are the means ± standard error of the mean (n = 6 per group): & p ≤ 0.05 respect to control; *p ≤ 0.05 respect to vehicle. c Clinical score in cuprizone model. Motor coordination and balance were evaluated twice a week from week 5 to week 9 using the rotarod test and expressed as seconds mice stay in the rotarod. Black diamonds, control with no cuprizone; black squares, cuprizone-fed mice; white triangles, cuprizone-fed vehicle treated mice; and white circles, cuprizone-fed and MTA treated mice. The graph represents the average time spent by mice on the rotarod at 18 rpm constant speed. * p ≤ 0.05 respect to cuprizone fed animals. & p ≤ 0.05 respect to vehicle cuprizone fed animals

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