Fig. 5
From: Methylthioadenosine promotes remyelination by inducing oligodendrocyte differentiation
Activation of signaling pathways involved in remyelination. Intracellular ERK1/2 (a), STAT3 (b), JNK/SAPK1 (c) and ATF2 (d) phosphoproteins measured in xMAP assays (Luminex) of mouse cerebellum cultures exposed to lysolecithin (0.5 mg/ml) and treated with methylthioadenosine (MTA, 192 μM) or the placebo at 9, 13 and 17 DIV. Samples for the phosphorylation assay were obtained 30 min after the last administration of MTA or the placebo. Results are the mean of three independent experiments with 10 slices per condition in each experiment. Gray, Control samples without lysolecithin; Black, Lysolecithin samples treated with placebo; White, Lysolecithin samples treated with MTA; * p < 0.05 and ** p < 0.01 with respect to the samples that received the placebo