Fig. 6

Modulation of signaling pathways associated with remyelination and cell survival. A Proportion of intracellular phospho-STAT-3 (Tyr705/Ser727) measured in xMAP assays of cerebellar cultures exposed to lysolecithin (0.5 mg/ml) and treated with MTA (192 μM) or the STAT3 inhibitor LLL12 (5 μM) for 4 days. The cells were processed 30 min after MTA or LLL12 exposure on the last day of experiment: *P < 0.05, statistical differences respect to control without lysolecithin; P < 0.05, statistical differences respect to Lysolecithin + LLL12 (no MTA). B CNTF gene expression in cerebellar organotypic cultures exposed to lysolecithin (0.5 mg/ml) and treated with MTA (192 μM) or LLL12 (2.5μM or 5μM): *P < 0.05, statistical differences relative to the control without lysolecithin; & P < 0.05, statistical differences relative to Lysolecithin + LLL12 (no MTA). C Immunohistochemistry for GFAP (red) and CNTF (green) in mouse cerebellar organotypic cultures exposed to lysolecithin and treated with MTA (192 μM) 4 d after treatment. Scale bar: 50 μm. Results are the mean of three independent experiments with 10 slices per condition in each experiment