Fig. 7

Role of methylthioadenosine in CNTF production and STAT-3 phosphorylation in astrocytes. a CNTF gene expression in primary cortical mouse astrocytes exposed to LPS (10 μg/ml) overnight and treated for 24 h with methylthioadenosine (MTA, 192μM) or the placebo on the following day. b CNTF protein measured by ELISA in supernatants of primary cortical mouse astrocytes exposed to LPS (10 μg/ml) overnight and treated for 24 h with MTA (192μM) or placebo the following day. c Intracellular phospho-STAT-3 (Tyr705/Ser727) levels measured in xMAP assays carried out on primary cortical mouse astrocytes exposed to LPS (10 μg/ml) overnight and treated with MTA (192μM) or placebo for the next 5 days (samples collected 15, 30 min or 1 h after the last treatment was given): * p < 0.05, statistical differences relative to the controls that did not receive LPS; p < 0.05, statistical differences relative to the cells that received LPS but not MTA. Results are the mean of two independent experiments