Hands on Alemtuzumab-experience from clinical practice: whom and how to treat
© The Author(s). 2016
Received: 11 January 2016
Accepted: 24 May 2016
Published: 2 August 2016
Alemtuzumab is a monoclonal antibody, which was recently approved for the treatment of active relapsing remitting multiple sclerosis. Its main mechanism of action is based on targeting CD52, an antigen of unknown function which is found on B and T lymphocytes, leading to depletion followed by repopulation of these cells. The high efficacy of Alemtuzumab in controlling relapsing remitting MS has been shown in several clinical trials. This new therapy approach is associated with a specific side effects profile requiring regular longterm monitoring. The most important side effects are infusion-associated reactions, a slight increase of infections as well as autoimmune events in almost one third of treated patients.
Based on two years of clinical experience in Germany, this review covers the first steps with the careful patient selection to be treated with Alemtuzumab over the preparation steps and the infusion courses up to the longterm monitoring after Alemtuzumab treatment.
In September 2013, Alemtuzumab was approved in Europe as an additional therapeutic option for active relapsing-remitting Multiple Sclerosis (RRMS). Since its synthesis in the early eighties as the first humanized monoclonal antibody, Alemtuzumab therapeutic potentials were explored for many diseases (eg. Graft versus host disease, transplantation, vasculitis, rheumatoid arthritis, immunologically mediated cytopenia) [1, 2]; it is the first depleting monoclonal antibody which was licensed for the treatment of RRMS. The mechanism of Alemtuzumab differs from immunmodulating and other immunosuppressive treatments in its fundamental approach, which consists of at least two courses of lymphocyte depletion followed by cellular repopulation. The reconstitution of lymphocytes is thought to be of particular importance not only for the beneficial clinical effects, but also for the long-term side effects . Alemtuzumab is introducing a new therapeutic concept of an induction-like treatment strategy, but without the need of a maintenance treatment in the follow up in most of the treated patients. Such concepts are till now not well established in our clinical practice. Furthermore, other highly efficacious treatment options such as Natalizumab and Fingolimod are available. No clinical trials have directly compared Alemtuzumab with these medications making the clinical decision not straight forward.
Although the role of Alemtuzumab in RRMS was investigated in three active-controlled clinical studies [4–6], using it in clinical practice can be quite challenging as several questions are still to be answered: how to define patients who will most likely profit from Alemtuzumab? Where does Alemtuzumab stand in MS treatment scenario? And then if Alemtuzumab is to be given, how should we switch patients from other specific MS treatments to Alemtuzumab? What other MS medications could be given following Alemtuzumab? How could the infusion management be optimized? Which side effects can be expected and how should they be handled? What is the most applicable monitoring strategy in clinical practice?
The aim of this review is to summarize our experience with Alemtuzumab in clinical practice of the last two years following a structured approach starting with treatment selection followed by preparation and infusion management up to the longterm monitoring.
The innovative treatment concept of alemtuzumab
Alemtuzumab is a monoclonal antibody that selectively binds to CD52, which is a surface glycoprotein molecule of unknown function . It is expressed on the surface of T- and B-cells and other cell populations but not on the hematological precursors . Alemtuzumab infusion results in rapid depletion of the CD52 positive cells , leading to lymphopenia, which lasts for several years . While B-cells recover relatively quickly within a range of 3–8 months, the repopulation of T cells occurs more slowly, as it needs up to 3 years and in another study up to 60 months for CD4+ and 30 months for CD8+ T cells in average [11, 12]. The experimental data using humanized CD52 mice have shown, that the depletion of lymphocytes upon anti-CD52 treatment was predominant in peripheral circulation, while the number of those cells was clearly less affected in spleen, thymus, bone marrow and lymph nodes [13, 14]. This may represent an explanation for the unexpected low rate of infections in patients receiving Alemtuzumab. As mentioned before, the depletion of CD52-positive cell is followed by a recovery phase that can vary between treated patients. However, it was shown that different repopulation kinetics among patients was not able to predict the risk of MS reactivation [14–16].
This process is considered as part of the reprogramming of the dysregulated immune system. Some authors suggest that the observed prolonged effect of Alemtuzumab lies in the slow reconstitution of T lymphocytes after the initial depletion, and not in the depletion itself .
The radical approach of Alemtuzumab which leads to its prolonged therapeutical effect is actually also associated with the well-described side effects. In addition to the infusion-associated reactions (IAR), which will be described in more details later, the increased incidence of antibody-mediated autoimmune diseases upon Alemtuzumab therapy is remarkable . Taking into consideration the previously described mechanism of immune cells depletion and the potential side effects, the need arises to find appropriate criteria that are useful for patient selection.
No biomarker to detect predisposition for secondary autoimmunity has been found yet. IL-21 was previously described as a promising candidate but these results could not be reproduced in another following study, which was attributed to the use of different kits [18, 19].
Different treatment strategies
Alemtuzumab therapy consists of two courses with 12 months in between. In spite of this non-frequent intermittent administration of the medication, this approach guarantees a long-term stabilization of the disease activity and to achieve the therapeutic effect, keeping in mind that Alemtuzumab infusions should take place under close monitoring. Until now, all available highly-efficacious therapies as Natalizumab and Fingolimod follow a continuous treatment strategy. After stopping these drugs, disease activity is returning or even overshooting as it has been described for Natalizumab [20, 21] and also even in fewer cases for fingolimod [22–25]. On the other side, treatment switches are easier to perform if a more or less reversible treatment as Fingolimod or Natalizumab is applied. The disadvantage is that the use of other treatment approaches after Alemtuzumab is more complicated due to the effects of irreversible depletion and repopulation, on the other side patient is practically off treatment after the second Alemtuzumab infusion. The rapid onset of Alemtuzumab treatment effects can be explained by depletion of both cells of the acquired and innate immune system, including the impairment in activation as well as cytokine release .
Patient selection: who is the right patient for Alemtuzumab?
According to the European Medicines Agency (EMA), Alemtuzumab is approved for the treatment of patients with active RRMS , whereas disease activity is defined by clinical and/or imaging criteria. According to the criteria for assessment of MS activity proposed by Lublin and colleagues , a patient with RRMS would be considered as having an active course if a clinical relapse took place or the brain MRI showed new Gadolinium-enhancing and/or the increase in size or number of T2 lesions. MRI monitoring should be done in regular annual intervals . In the clinical trials, the disease duration was limited to 5 years in treatment naïve and to 10 years in pretreated patients [4, 6] probably because Alemtuzumab was not able to cause improvement in SPMS patients in a small pivotal study . So in our opinion, the benefit of the patients regarding Alemtuzumab treatment is optimal if significant inflammatory signs are present clinically and by MRI. So Alemtuzumab is not indicated for patients who do not show any disease activity, and it is also not considered as a standard alternative for patients who are stable on current therapy.
It should be emphasized that for MS patients with high disease activity, highly efficacious therapy options such as Alemtuzumab should be used early within a “window of opportunity” . That implies that the optimal timing would be in the presence of significant clinical and MRI inflammatory activity without significant disability . Therapeutical efficacy of Alemtuzumab is most prominent in early stages of disease by reducing inflammatory activity to counteract the development and accumulation of disability [11, 35]. Patients presenting with an EDSS value up to 3–4 still potentially have predominant inflammatory processes, while irreversible damage is increasingly assumed to be associated with higher EDSS values . Accordingly, Alemtuzumab is most appropriately to be used before an EDSS score of 4 is reached. This should not be interpreted that patients with higher EDSS scores can not benefit from treatment with Alemtuzumab, as the indication should be considered based on clinical and/or radiological disease activity Actually in some patients Alemtuzumab is used as “last option” treatment as many treatments before have failed. The efficacy of Alemtuzumab as last option treatment strategy has to be investigated by defined cohort or real world studies [37, 38].
In addition to the disease activity as a determining factor in the therapeutic decision, other factors, such as patient personality and cognitive skills, should be taken into consideration. Because of necessary longterm monitoring patient’s discipline is demanded, as Alemtuzumab treatment is potentially associated with severe adverse effects which have to be identified early .
Keeping in mind that different forms of MS are mediated by different pathological processes, possibly implicating different therapeutical approaches, the diagnosis and classification of MS should be definitely confirmed. Neuromyelitis optica (NMO) which was long been considered as a special form of MS, is associated with different etiology. Alemtuzumab is not considered as a first line treatment option in this disease, as several case reports showed a worsening of NMO under Alemtuzumab treatment [40–42].
As no previous studies have yet compared the efficacy of Alemtuzumab directly with other highly active therapies, such as Natalizumab or Fingolimod, the clinical decision to choose one of the above mentioned medications is largely based on other factors. That would include on top of the list the potential side effects of each drug versus the expected benefit. Additionally the relative and absolute contraindications should be carefully assessed to choose the most suitable drug. For example, patients with positive anti-JCV status represent high-risk group for PML development on longterm Natalizumab treatment . For female patients in child-bearing age, Alemtuzumab can be the choice, but the it should be planned 4 months after the last infusion .
Absolute and relative contraindications of Alemtuzumab
Hypersensitivity to the active substance, or to any of the excipients.
Severe active infections. Additionally, this drug should not be used in the presence of active chronic or recurrent bacterial or viral infections, such as TB, HCV. HBV or HIV.
Pregnancy or lactation.
Blood coagulation disorders such as dysfibrinoginemia, factor IX deficiency, hemophilia, von Willebrandt disease, or anti-coagulation therapy.
Significant infection liability, such as aspiration susceptibility or frequent urinary tract infection.
Malignant diseases in the patient history, which is not curatively treated.
Negative Varicella-zoster IgG status
Severe liver or kidney insufficiency
Children under 18 years of age
Patient preparation: pretreatment
Prior therapy with Natalizumab represents a special situation as the switching protocol depends on the actual PML risk which is closely linked to the JCV status and treatment duration . The challenge is to find a way between returning disease activity after Natalizumab and PML risk. Because of the irreversible and acutely immunosppressive mechanism of action of Alemtuzumab, a carryover PML after natalizumab treatment which has been described occurring even up to 6 months after Natalizumab cessation has to be excluded before treatment start [46, 47]. So if there is only a low PML risk which means treatment duration with Natalizumab less than 2 years and/or negative anti-JCV antibodies, a washout period between two and three months is considered sufficient until pharmacodynamic effects of Natalizumab have disappeared. MRI and possibly CSF analysis should be performed as part of the work up as described later. For patients with higher PML risk (JCV antibody positive and treatment duration > 2 years) we recommend a stepwise approach. 3 months after last infusion first MRI should be performed to exclude PML and monitor return of disease activity. If no MRI activity is present, follow up MRI will be performed 4 weeks later which will be repeated until 6 months. If there is MRI activity (or clinical activity) present, CSF analysis with JCV DNA and cell count should be performed before Alemtuzumab is started. This procedure has been applied many times in our clinical practice and can avoid return of clinical activity and makes PML much more unlikely. This means that a strict 6 months washout period cannot be applied in all PML high risk patients on Natalizumab. It is crucial to implement an individual approach for the switch of natalizumab to Alemtuzumab. As irreversible, cell depleting therapy, a carry over of a Natalizumab-associated PML has to be avoided in potential Alemtuzumab patients. In our hands, identifying patients with MRI activity was mostly sufficient to avoid clinical rebound activity as Alemtuzumab demonstrates a rapid onset of action. After immunosuppressive therapies, such as Azathioprine, Cyclosporine A, Mitoxantrone or Cyclophosphamide, which are rarely used nowadays, wash-out period of 3–6 months is necessary in addition to physiological white blood cell counts. Nevertheless patients after immunosuppressive pretreatment are not the ideal patients although case reports have reported beneficial effects .
In general, it should be kept in mind that these recommendations are general. In everyday clinical practice we are confronted with special individual patient cases that may require deviation from the general recommendations. These adjustments are justified depending on the individual disease activity and progression, and it is advisable to document the reason for the different approach appropriately .
Patient preparation: preparatory steps
Recommended lab testing before Alemtuzumab treatment/retreatment
Differential blood count, and optionally an immune status depending on the pretreatment and on the number of lymphocytes
Liver panel including SGOT, SGPT, GGT, bilirubin
Kidney panel including Creatinine, GFR
Thyroid panel including TSH, T3, T4
Infection status including Syphilis, VZV, HIV, hepatitis serology, TB test (eg. Quantiferon test)
Coagulation panel including INR, PTT
Urine panel including quantitative urinalysis by sediment microscopic analysis
HPV screening and pregnancy test in women
Infection screening panel including CRP, BSG
MRI of the complete neuroaxis (not older than three months) should be performed as documentation for baseline status before Alemtuzumab start. All the above-mentioned tests should be accomplished and managed before the first treatment course; and these safety investigations should be repeated prior to the commencement of the second treatment course. There are no specific requirements regarding lymphocyte or leukocyte cell counts which have to be reached before second Alemtuzumab course.
Women with childbearing potential should be informed to use an effective contraception during treatment phase and four months thereafter.
Especially for patients who have a history of neither chicken pox infection nor of previous vaccination against Varicella-Zoster virus, it is obligatory to measure its antibodies level before initiating Alemtuzumab treatment. We recommend VZV testing in all patients before Alemtuzumab application. For those who are VZV antibody negative the vaccination should be given, followed by measuring the VZV antibody titer four to six weeks after VZV vaccination. As vaccinations may be complication by the depleting-repleting mechanism of action vaccination status should be checked and necessary vaccinations should be performed before first Alemtuzumab application.
Before first Alemtuzumab treatment, patients should be informed about potential side effects and the need for regular monitoring which is mandatory for a beneficial risk-beneftit ratio of Alemtuzumab.
Alemtuzumab infusion management
Recommended infusion protocol
Concomitant medication: The evening before the 1st Alemtuzumab infusion for all infusion stages, orally:
– H1 blockade eg. Cetirizine 1-0-1 (non-sedating H1 antihistamine)
– H2 blockade eg Ranitidine 300 mg 1-0-1 (H2 receptor antagonist)
– Herpes prophylaxis eg. Aciclovir 200 mg 1-0-1 (for HSV/VZV prophylaxis; for at least 4 weeks)
1st year: infusion 5 days (Mon.-Fri.)
2nd year infusion 3 days (3 consecutive days)
Cardiovascular (BP, heart rate) + Body temperature (1×/30 min) Monitoring
– 1st day of infusion course: Perform pregnancy test in female patients with childbearing potential
– Insertion of peripheral permanent port (can also be used for other medications)
1. 60 min. before Alemtuzumab infusion:
a) Methylprednisolone 1 g i.v. as short infusion at the first 3 infusion days
b) Paracetamol 1 g. i.v. as short infusion at all infusion days
2. Alemtuzumab 12 mg i.v. via infusion pump (12 mg per day on each of 5 (or 3) days), is prepared as ready-to-use solution supply (volume 112 mL). Storage time of the infusion 8 h after preparation.
- Target infusion period about 4 h (in case of side effects-especially at the start of the infusion-reduction of the infusion speed)
3. NaCl infusion 100 mL over 30 min (infusion pump), to flush residual medication out of the infusion line (tube)
Also to note:
– Monitoring until 2 h after infusion
– Drinking adequate liquids (at least 2 l/d)
– Availability of trained physician and medical personnel during the entire period of the infusion
Potential acute adverse events
1. Anaphylaxia/anaphylactic shock (quite rare)
2. Infusion-associated reactions (IAR):
– Erythema, urticaria, pruritis, (fever, headache, fatigue)
– Intensified neurological symptoms (Uhthoff Phenomenon!)
➔ STOP infusion and inform physician immediately!
➔ Fenistil 1 A (4 mg) i.v.
➔ In case of insufficient effect ➔ 250 mg prednisolone i.v.
Depending on severity of the IAR, continue infusion, but more slowly.
Routine follow-up care
1. Aciclovir 200 mg 1-0-1 for 4 weeks from the first day of the infusion
2. Antihistamine (e.g. cetirizine 1-0-1) for an additional week
3. Paracetamol standby (if headache or fever appear)
First check-up appointment MS centre 4 weeks after infusion.
Procedural measures/follow up checks
– Adequate infection protection including adjusting diet (no cheese from raw milk, raw fish/raw meat)
– During the infusion course, avoid stomach-irritating foods such as fruit acids, carbonates, sharp or strong-smelling foods, no sodium glutamate-“bland diet”
– If applicable, safe contraception methods for at least 4 months after the infusion course
– Due to the known potential side effects, the regimen of follow-up check-ups organised and implemented by the MS centre must be strictly observed
– Patients are instructed to observe a low threshold for making an acute appointment in case of infections. In this respect, the reduced immune competence and reduced lymphocyte count must be considered, especially in the first months after infusion, and a complete focus screening must be conducted.
– The patient should be informed of the symptoms of possible side effects (eg. ATP, glomerulonephritis), and should inform the treating physician of them. An early appointment with the physician should also be made in case of infection.
The day before Alemtuzumab infusion, H1 and H2 blockade should be initiated which could be applied beyond the infusion week if necessary [30, 50]. An anti-pyretic, given 30–60 min before infusion, may help to reduce the infusion reaction.
In addition, a short infusion of methylprednisolone (1 g) is recommended for the first three days of treatment. Cytokine release and lymphocyte activation could account for such infusion-associated reactions [51, 52]. Induction of serum cytokines could be due to cross-linking of NK cells in addition to cell lysis . Pivotal studies not using standardized infusion procedure and methylprednisolone pre-treatment demonstrated higher levels of serum cytokines and significant more IARs after initial alemtuzumab infusions . Pre-and concomitant methylprednisolone treatment incorporated into the standard infusion protocol may attenuate cytokine release syndrome and help reduce infusion-associated reactions to enhance patient tolerance . This protocol is based on the procedure in pivotal studies. However, in deviation from this study experience, the steroid infusion may be adjusted depending on the individual situation and the patient’s response. This mean extension over 5 days or potential pretreatment one day before Alemtuzumab infusion which is actually investigated in a clinical trial. If the Alemtuzumab course could not be completed over the consecutive 5 days, if the course has to be interrupted because of eg. acute infection with fever, the remaining Alemtuzumab infusions should be performed as soon as possible in the next weeks. In this case, methylprednisolone should be applied before all remaining Alemtuzumab infusions.
Alemtuzumab infusion should be started after methylprednisolone infusion. Mixing these preparations is not allowed, as this can lead to precipitation of the drug. Alemtuzumab itself is diluted immediately before the infusion in 100 ml of 0.9 % sodium chloride solution or 5 % glucose solution (chemically stable at 2–8 °C for up to 8 h). Other infusions could be applied using the same intravenous port as Alemtuzumab, a paravenous infusion is not critical as Alemtuzumab is a monoclonal antibody and not a chemotherapy. The infusion-associated reactions (IAR) occur in up to 90 % of patients treated with Alemtuzumab . Skin reactions are observed predominantly. With mild symptoms, symptomatic treatment with anti-histamergic treatments or anti-pyretics/anti-inflammatory treatments and slower infusion rate are usually sufficient. In case of severe reactions such as fever, urticaria, atrial fibrillation, nausea, chest discomfort or hypotension, the infusion should be stopped immediately. Because of these potential acute infusion-associated reactions, anaphylaxis therapy should be kept ready although the reaction to Alemtuzumab is generally not anaphylactic, so that the infusion can be further continued. The observation for two hours after the end of the infusion is necessary. In our hand, monitoring of the patient’s temperature seems to be an effective method for early detection of an infusion-associated reaction in everyday practice . It is therefore recommended to monitor and document the body temperature every half hour. Heart rate and blood pressure should also be measured every one hour. In addition to this reaction, reappearance of old neurological deficits, which occurred in the context of past relapses, can also occur, which it is caused by the release of cytokines due to cell depletion in a process similar to Uhthoff effect (Cytokine Uhthoff syndrome) . The highest drug level is reached after the last infusion . However, even if this falls in the next few days, its effect with regard to lymphocyte depletion goes on, so that it is still possible to experience reactions related to cells depletion .
Alemtuzumab treatment is associated with an increased risk for the development of autoimmune diseases, for this reason a regular monitoring is indicated to allow for an early recognition and thereby early treatment of these diseases [10, 17, 19, 54–57]. Most of the autoimmune phenomena have a beneficial prognosis if they are identified early and anti-inflammatory treatment is started early. So monthly check-ups should take place up to 48 months after the last infusion, including a complete differential blood count to detect the development of Alemtuzumab-induced thrombocytopenic purpura (ATP) [58, 59]. In addition, monthly monitoring of serum creatinine levels and urine tests with microscopy for an early detection of possible glomerulonephritis are necessary . Thyroid function test using the TSH value should also be performed every three months. In pregnant patients with special interest in thyroid monitoring monthly TSH testing is recommended [55, 57, 60].
The monitoring does not need to be performed by the treating neurologist. Good experiences are reported in terms of the cooperation with general practitioners, who can perform or arrange for the check-ups. However, it is essential that the treating neurologist ensures that the control tests are carried out according to previous specifications and the obtained results are evaluated. Treating neurologist has to organize and supervise monitoring after Alemtuzumab treatment. Patients should be clearly informed not to wait for problems to happen, but immediately and regularly communicate with their physician. Modern communication tools as email reminders, smartphone could be nicely implemented in the patient management .
Collecting real world data in an alemtuzumab registry
Although the clinical experience with the agent has substantially increased since market introduction, there are only few reports yet on the long-term use of the drug in the routine of physicians and MS centers [10, 16, 44]. That is why real world data about Alemtuzumab data should be collected as the German non-interventional long-Term study foR obsErvAtion of Treatment with Alemtuzumab (TREAT-MS) which is documenting physician and patient experience in daily clinical practice for six years . Data for this non-interventional study will be collected on a widely unselected patient population eligible for Alemtuzumab treatment. It is expected that compared to the clinical studies, patients with more concomitant diseases and/or more concomitant medications will be documented. Together with the high patient numbers and long follow-up period, a substantial number of patient years will be documented and the option for relevant subgroup analyses provided .
As infections can be facilitated by cellular depletion of the adaptive and innate immune system together and infusion-associated reaction symptoms like fever, headache, and fatigue may mimic infectious signs, routine blood analysis is usually a suitable tool to identify infectious conditions. But laboratory monitoring during infusion therapy is not needed, and it can even be affected by cell depletion-related artifacts . During the the first infusion week, rapid cellular depletion and impairment of activation of different adaptive and innate immune cell subtypes take place accompanied by marked serum cytokine increases. Nevertheless, these acute Alemtuzumab-mediated effects are assumed to lead to several effects observable by standard blood testing, such as the non-infectious increase in leukocyte count, CRP, and PCT, which are of particular importance in identification and monitoring of infectious conditions. Furthermore, transient elevation of liver enzymes, thrombocytopenia, and TSH modulation are demonstrated. So we recommend clinicians to be aware of clinical symptoms and vital data to initiate supportive analysis rather than standard laboratory testing within the first alemtuzumab treatment week .
In general, patients should also be advised to avoid the consumption of raw foods such as raw milk products because of possible contamination with listeria . The ability of Alemtuzumab to deplete dendritic cells as well may explain cases of Listeria meningitis linked to the initial Alemtuzumab infusions [9, 67, 68]. Listeria meningitis infection may be facilitated by immune cell depletion in the adaptive as well as the innate immune system, possibly by an outburst of a pre-existing, clinically silent and CD8+ T cell controlled infection due to cellular depletion and activation blockade. These findings highlight the relevance of certain infections, which could be promoted by the depletion and blockade of innate immune subsets, that clinicians should be aware of within the first days after initial Alemtuzumab infusion.
Patients who have acute or chronic infections should not start with Alemtuzumab therapy. As mentioned above the presence of acute infections under Alemtuzumab therapy demands immediate diagnostic and therapeutic procedures. Taking into consideration that human papilloma virus infections (including cervical dysplasia) were described, it is obligatory for female patients to perform an annual HPV-screening with cervical cytology, especially when no former HPV vaccination was given.
Due to the observed increased rate of herpes infections, prophylaxis with acyclovir is recommended . In the phase 3 clinical studies, this prophylactic regimen was introduced not from the beginning on [4, 6, 59]. But the patients receiving 200 mg of acyclovir twice daily or an equivalent for this purpose demonstrated less herpetic infections. Prophylaxis should begin on the first day of each treatment phase and should be maintained throughout the course of treatment for at least for 1 month. It should be noted that acyclovir can cause several immunological phenomena including rash itself, which has to be differentiated from IARs and could be improved by the transition to a newer treatments, such as Valacyclovir, as an alternative.
In the approval trials infections were moderately increased in Alemtuzumab group compared with interferon group, represented mainly by mild to moderate infections in the upper respiratory tract, gastroenteritis and urinary tract infections . This is a completely different profile compared to hematological patients treated by higher doses of Alemtuzumab demonstrating specific opportunistic infections of immunosuppression . In MS patients, infections were not prolonged and receded under traditional medical therapy. Serious or opportunistic infections were not noticed to be increased. Pre-existing chronic diseases, such as tuberculosis, can be reactivated. No data are available regarding reactivation of controlled chronic HBV/HCV infections. In a recent observation period of 7 years no change in the malignancy rate has been detected . As the thyroid is carefully investigated because of thyroid autoimmunity by eg. ultrasound, a rise in the incidence of thyroid papillary carcinoma diagnosis may be the consequence suggesting overdiagnosis .
Autoimmune adverse events
The development of autoimmune thyroid diseases is of high importance as it can be detected in more than 35 % of patients treated with Alemtuzumab in the 4 years after first Alemtuzumab infusion . Both hypo- and hyperthyroidism may occur, and the development of Graves’ disease was highly prevalent .
The peak of incidence is reported to be in the third year after the first dose of Alemtuzumab. Most events are of mild to moderate severity and can respond to conventional therapies. However, surgical intervention may be needed in less than 1 % of patients. The occurrence of thyroids side effects requires careful assessment of the clinical status of the patient and the necessity of medical and/or surgical therapies before continuing Alemtuzumab treatment. According to data derived from clinical studies, the baseline value of anti-TPO antibodies before starting alemtuzumab could not predict the development of thyroid side effects. Almost 80 % of patients who manifested with thyroidal problems after alemtuzumab therapy, showed negative anti-TPO antibody status at the beginning of the study .
Acute ITP has occured in ab to 2 % of the treated patients in clinical MS-studies within 14–36 months after the first exposure to alemtuzumab. ATP can manifest clinically with higher tendency to bruises, petechias, spontaneous mucosal bleedings (epistaxis, hemoptysis), severe or irregular menstrual bleeding or symptoms of anemia due to blood loss including serious hemodynamic complications. Complete blood count including platelet value should be performed monthly over at least 48 months after the last alemtuzumab infusion. The platelet control should be performed weekly in case that platelet count drops to less than 30 % of its initial value or below the normal range, and hematological consultation should be ordered when the platelet number is less than 100.000 GPt/l.
Normally the acute treatment of thrombocytopenia is usually low to high dose corticosteroids, it does not tend to develop a chronic condition. Reports about continuing alemtuzumab therapy after the development of ATP are limited and giving a new alemtuzumab infusion should be assessed on an individual basis .
Nephropathies and individual cases of Goodpasture’s syndrome with renal and pulmonary infection were reported in 0.3 % of the patients [44, 74]. Nephropathies including anti-glomerular basement membrane (anti-GBM) disease, occured in 0.3 % of the patients in clinical MS-studies up to 39 months after the last administration of Alemtuzumab. These cases were early recognized by clinical and laboratory control, so that they could follow favorable course after effective treatment. The development of anti-glomerular basement membrane (anti-GBM) disease without suitable monitoring had led to renal failure, which in case of delayed treatment led to dialysis and/or kidney transplantation. Such side effects can be detected by laboratory monitoring through the presence of hematuria, proteinuria and/or creatinine elevation. Consequently, an urgent specialized nephrological consultation will be necessary.
The efficacy of vaccines during and closely after Alemtuzumab application can be limited. That is why it is recommended to check the success of the vaccinations by determining their respective titers. The use of attenuated living vaccination should be avoided. Patients should fulfill the vaccination requirements at least six weeks before starting the Alemtuzumab therapy. It should be emphasized that for patients treated with Alemtuzumab the flu-vaccine should be delayed at least six months after the last infusion; however this vaccination can be repeated in case the first vaccination was not efficient.
One pilot study showed that for patients treated with Alemtuzumab the humoral immunological response after different vaccinations was not different from that detected in respective controls [6, 75], implying that the immune competence is restored after lymphocytes depletion induced by Alemtuzumab therapy. This is in line with the data derived from clinical studies which demonstrated low incidence of opportunistic infections following Alemtuzumab infusions. However this study was small and only 5 patients were vaccinated within the first 6 months after Alemtuzumab [16, 76, 77].
Moreover different studies suggested that the long lasting lymphopenia is neither associated with higher incidence of infections nor with higher activity of MS compared with accelerated lymphocyte reconstitution [12, 15, 78].
No safety data are available till now regarding the potential influence of Alemtuzumab on male fertility. The concerns regarding potential effect on fertility remains not well investigated, as the target antigen CD52 is also found in the tissues of the reproductive system.
Alemtuzumab is contraindicated during pregnancy and lactation, as animal studies have shown reproductive toxicity because Alemtuzumab, as well as human immunoglobulins, can cross the placenta. The same applies during lactation as they can be transferred by breast milk, and the risk to the infant is unknown. Additionally, the increased incidence of autoimmune diseases such as thyroid disease, particularly by the use of Alemtuzumab in women of childbearing age represents potential concern .
The development of autoimmune diseases had been observed during the study period within a maximum in the third year after first infusion followed by a decrease in risk . So it makes sense to plan for pregnancy either after this period has passed or before the third year which means 4 months after the second infusion, and then profit from the prolonged high efficiency without the need for additional medication.
Treatment following the 2 Alemtuzumab courses
The majority of MS patients treated with Alemtuzumab do not need additional Alemtuzumab infusions or different MS treatments in the first 5 years after Alemtuzumab treatment. In a recent analysis for ECTRIMS 2015, 68 % of CARE-MS1 and 60 % of CARE-MS2 have not received additional Alemtuzumab infusions. 22.1 % resp. 8.0 % of CARE-MS1 and 28.8 % resp. 9.9 % of CARE-MS2 patients have received one resp. two Alemtuzumab retreatments. In both clinical trials, the threshold for Alemtuzumab retreatment was quite low with one relapse activity or two new T2 lesions resp. one new Gd + lesion in the follow up MRI. If the treatment strategy of Alemtuzumab has been selected after careful consideration, this selected strategy should be generally maintained which means that retreatments should be applied at least once or twice if needed. Every retreatment should be accompanied with careful consideration about retreatment, disease activity, response to Alemtuzumab and additional treatment options. If the patient seems to be an Alemtuzumab-non-responder, alternative treatment strategies should be considered as eg. Fingolimod or B-cell depleting therapies.
In the clinical trials, 2.3 % resp. 7.6 % of the CARE-MS 1 resp. 2 patients have been treated with other MS treatments in the first 5 years after Alemtuzumab start. The majority of these 96 out of 1496 patients used baseline medication (interferon and GA), 10 % each were treated with Fingolimod and Natalizumab. Switching after Alemtuzumab to Interferon-beta or Glatiramer Acetate can be done quite quickly after the last Alemtuzumab infusion after excluding relevant lymphopenia especially for interferon treatment. There is not a lot of experience with the new oral medications Teriflunomide and Dimethylfumarate. Especially with Dimethylfurmarate, the previous immunosuppression with Alemtuzumab has to be considered. By switching to Natalizumab it should be kept in mind that Alemtuzumab leads to significant long-lasting changes in the adaptive immunity cells. As Alemtuzumab works as an immunosuppressive, changing to Natalizumab is associated with higher risk for PML development especially in patients with positive JCV antibodies. The better treatment option of highly active MS treatments which has already been used in our center is Fingolimod. Because of the individually very different prolonged effect of Alemtuzumab on the adaptive immunity, it is not possible to give a general waiting period after the last Alemtuzumab infusion. A complete immune status can be helpful including differential blood count, CD4+ T cells, CD8+ T cells, B cells, NK cells.
Alemtuzumab is a highly efficacious disease-modifying therapy which offers a new treatment principle for the treatment of relapsing-remitting MS. It shows clear benefits on relapses, disability outcomes, and freedom from clinical disease and magnetic resonance imaging activity. Infusion-associated reactions are common with Alemtuzumab, but rarely serious. Infection incidence was elevated with Alemtuzumab in clinical studies; most infections were mild or moderate in severity. Autoimmune adverse events occurred in approximately a third of patients, manifesting mainly as thyroid disorders, and less frequently as immune thrombocytopenia or nephropathy. A comprehensive monitoring program lasting at least 4 years after the last alemtuzumab dose allows early detection and effective management of autoimmune adverse events. Further experience with alemtuzumab in the clinic will provide needed long-term data.
LH and TZ designed and drafted the manuscript. JE and KT added important contact. All authors read and approved the final manuscript.
Tjalf Ziemssen has received reimbursements for participation in scientific advisory boards from Bayer Healthcare, Biogen Idec, Novartis Pharma AG, Merck Serono, Teva, Genzyme, and Synthon. He has also received speaker honorarium from Bayer Healthcare, Biogen Idec, Genzyme, Merck Sharp & Dohme, GlaxoSmithKline, Novartis Pharma AG, Teva, Sanofi Aventis, and Almirall. He has also received research support from Bayer Healthcare, Biogen Idec, Genzyme, Novartis Pharma AG, Teva, and Sanofi Aventis. Katja Thomas received honorarium from Novartis and Bayer. Lina Hassoun and Judith Eisele have no disclosures.
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- Coles A, Deans J, Compston A. Campath-1H treatment of multiple sclerosis: lessons from the bedside for the bench. Clin Neurol Neurosurg. 2004;106:270–4.View ArticlePubMedGoogle Scholar
- Kousin-Ezewu O, Coles A. Alemtuzumab in multiple sclerosis: latest evidence and clinical prospects. Ther Adv Chronic Dis. 2013;4:97–103.View ArticlePubMedPubMed CentralGoogle Scholar
- Cox AL, Thompson SAJ, Jones JL, Robertson VH, Hale G, Waldmann H, et al. Lymphocyte homeostasis following therapeutic lymphocyte depletion in multiple sclerosis. Eur J Immunol. 2005;35:3332–42.View ArticlePubMedGoogle Scholar
- Cohen JA, Coles AJ, Arnold DL, Confavreux C, Fox EJ, Hartung H-P, et al. Alemtuzumab versus interferon beta 1a as first-line treatment for patients with relapsing-remitting multiple sclerosis: a randomised controlled phase 3 trial. 2012.Google Scholar
- CAMMS223 Trial Investigators, Coles AJ, Compston DAS, Selmaj KW, Lake SL, Moran S, et al. Alemtuzumab vs. interferon beta-1a in early multiple sclerosis. N Engl J Med. 2008;359:1786–801.View ArticleGoogle Scholar
- Coles AJ, Twyman CL, Arnold DL, Cohen JA, Confavreux C, Fox EJ, et al. Alemtuzumab for patients with relapsing multiple sclerosis after disease-modifying therapy: a randomised controlled phase 3 trial. 2012.Google Scholar
- Watanabe T, Masuyama J-I, Sohma Y, Inazawa H, Horie K, Kojima K, et al. CD52 is a novel costimulatory molecule for induction of CD4+ regulatory T cells. Clin Immunol. 2006;120:247–59.View ArticlePubMedGoogle Scholar
- Xia MQ, Tone M, Packman L, Hale G, Waldmann H. Characterization of the CAMPATH-1 (CDw52) antigen: biochemical analysis and cDNA cloning reveal an unusually small peptide backbone. Eur J Immunol. 1991;21:1677–84.View ArticlePubMedGoogle Scholar
- Thomas K, Eisele JC, Rodriguez-Leal FA, Hainke U, Ziemssen T. Acute effects of alemtuzumab infusion in active relapsing remitting multiple sclerosis patients. Neurology: Neuroimmunology & Neuroinflammation. 2016;3, e228.Google Scholar
- Willis MD, Harding KE, Pickersgill TP, Wardle M, Pearson OR, Scolding NJ, et al. Alemtuzumab for multiple sclerosis: Long term follow-up in a multi-centre cohort. Mult Scler. 2015.Google Scholar
- Coles AJ, Cox A, Le Page E, Jones J, Trip SA, Deans J, et al. The window of therapeutic opportunity in multiple sclerosis: evidence from monoclonal antibody therapy. J Neurol. 2006;253:98–108.View ArticlePubMedGoogle Scholar
- Cossburn MD, Harding K, Ingram G, El-Shanawany T, Heaps A, Pickersgill TP, et al. Clinical relevance of differential lymphocyte recovery after alemtuzumab therapy for multiple sclerosis. Neurology. 2012;80:55–61.View ArticlePubMedGoogle Scholar
- Freedman MS, Kaplan JM, Markovic-Plese S. Insights into the Mechanisms of the Therapeutic Efficacy of Alemtuzumab in Multiple Sclerosis. J Clin Cell Immunol. 2013;4.Google Scholar
- Hu Y, Turner MJ, Shields J, Gale MS, Hutto E, Roberts BL, et al. Investigation of the mechanism of action of alemtuzumab in a human CD52 transgenic mouse model. Immunology. 2009;128:260–70.View ArticlePubMedPubMed CentralGoogle Scholar
- Kousin-Ezewu O, Parker RA, Tuohy O, Compston A, Coles A, Jones J. Accelerated lymphocyte recovery after alemtuzumab does not predict multiple sclerosis activity. Neurology. 2014;82:2158–64.View ArticlePubMedPubMed CentralGoogle Scholar
- Hill-Cawthorne GA, Button T, Tuohy O, Jones JL, May K, Somerfield J, et al. Long term lymphocyte reconstitution after alemtuzumab treatment of multiple sclerosis. J Neurol Neurosurg Psychiatr. 2012;83:298–304.View ArticlePubMedGoogle Scholar
- Cossburn M, Pace AA, Jones J, Ali R, Ingram G, Baker K, et al. Autoimmune disease after alemtuzumab treatment for multiple sclerosis in a multicenter cohort. Neurology. 2011;77:573–9.View ArticlePubMedGoogle Scholar
- Jones JL, Phuah C-L, Cox AL, Thompson SA, Ban M, Shawcross J, et al. IL-21 drives secondary autoimmunity in patients with multiple sclerosis, following therapeutic lymphocyte depletion with alemtuzumab (Campath-1H). J Clin Invest. 2009;119:2052–61.PubMedPubMed CentralGoogle Scholar
- Harding KE, Cossburn M, Robertson N, Compston A, Coles AJ, Jones JL. Predicting autoimmunity after alemtuzumab treatment of multiple sclerosis. J Neurol Neurosurg Psychiatr. 2014;85:795–8.View ArticlePubMedGoogle Scholar
- Sørensen PS, Koch-Henriksen N, Petersen T, Ravnborg M, Oturai A, Sellebjerg F. Recurrence or rebound of clinical relapses after discontinuation of natalizumab therapy in highly active MS patients. J Neurol. 2014;261:1–8.View ArticleGoogle Scholar
- Vellinga MM, Castelijns JA, Barkhof F, Uitdehaag BMJ, Polman CH. Postwithdrawal rebound increase in T2 lesional activity in natalizumab-treated MS patients. Neurology. 2008;70:1150–1.View ArticlePubMedGoogle Scholar
- La Mantia L, Prone V, Marazzi MR, Erminio C, Protti A. Multiple sclerosis rebound after fingolimod discontinuation for lymphopenia. Neurol Sci. 2014;35:1485–6.View ArticlePubMedGoogle Scholar
- Havla JB, Pellkofer HL, Meinl I, Gerdes LA, Hohlfeld R, Kümpfel T. Rebound of disease activity after withdrawal of fingolimod (FTY720) treatment. Arch Neurol. 2012;69:262–4.View ArticlePubMedGoogle Scholar
- Piscolla E, Hakiki B, Pasto L, Razzolini L, Portaccio E, Amato MP. Rebound after Fingolimod suspension in a pediatric-onset multiple sclerosis patient. J Neurol. 2013;260:1675–7.View ArticlePubMedGoogle Scholar
- Sempere AP, Berenguer-Ruiz L, Feliu-Rey E. Rebound of disease activity during pregnancy after withdrawal of fingolimod. Eur J Neurol. 2013;20:e109–10.View ArticlePubMedGoogle Scholar
- Havrdova E, Horakova D, Kovarova I. Alemtuzumab in the treatment of multiple sclerosis: key clinical trial results and considerations for use. Ther Adv Neurol Disord. 2015;8:31–45.View ArticlePubMedPubMed CentralGoogle Scholar
- Weber MS, Menge T, Lehmann-Horn K, Kronsbein HC, Zettl U, Sellner J, et al. Current treatment strategies for multiple sclerosis-efficacy versus neurological adverse effects. Curr Pharm Des. 2012;18:209–19.View ArticlePubMedGoogle Scholar
- Plavina T, Subramanyam M, Bloomgren G, Richman S, Pace A, Lee S, et al. Anti-JCV antibody levels in serum or plasma further define risk of natalizumab-associated PML. Ann Neurol. 2014;76:802–12.View ArticlePubMedGoogle Scholar
- Fenu G, Lorefice L, Frau F, Coghe GC, Marrosu MG, Cocco E. Induction and escalation therapies in multiple sclerosis. Antiinflamm Antiallergy Agents Med Chem. 2015;14:26–34.View ArticlePubMedGoogle Scholar
- Bayas A, Baum K, Bitsch A, Haas J, Hellwig K, Lang M, et al. One Year Alemtuzumab – What have we Learned in Clinical Practice? Exchange of Experience between Experts on Treatment of Multiple Sclerosis. Akt Neurol. 2015; 42(09):535-41.Google Scholar
- Lublin FD, Reingold SC, Cohen JA, Cutter GR, Sørensen PS, Thompson AJ, et al. Defining the clinical course of multiple sclerosis: the 2013 revisions. Neurology. 2014;83:278–86.View ArticlePubMedPubMed CentralGoogle Scholar
- Bermel RA, Naismith RT. Using MRI to make informed clinical decisions in multiple sclerosis care. Curr Opin Neurol. 2015;28:244–9.View ArticlePubMedGoogle Scholar
- Ziemssen T, De Stefano N, Pia Sormani M, Van Wijmeersch B, Wiendl H, Kieseier BC. Optimizing therapy early in multiple sclerosis: An evidence-based view. Multiple Sclerosis and Related Disorders. 2015;4:460–9.View ArticlePubMedGoogle Scholar
- Ziemssen T, Derfuss T, Stefano N, Giovannoni G, Palavra F, Tomic D, et al. Optimizing treatment success in multiple sclerosis. J Neurol. Springer Berlin Heidelberg; 2015;1–15.Google Scholar
- Coles AJ, Wing M, Smith S, Coraddu F, Greer S, Taylor C, et al. Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis. Lancet. 1999;354:1691–5.View ArticlePubMedGoogle Scholar
- Leray E, Yaouanq J, Le Page E, Coustans M, Laplaud D, Oger J, et al. Evidence for a two-stage disability progression in multiple sclerosis. Brain. 2010;133:1900–13.View ArticlePubMedPubMed CentralGoogle Scholar
- Le Page E, Deburghgraeve V, Lester M-A, Cardiet I, Leray E, Edan G. Alemtuzumab as rescue therapy in a cohort of 16 aggressive multiple sclerosis patients previously treated by Mitoxantrone: an observational study. J Neurol. 2015;262:1024–34.View ArticlePubMedGoogle Scholar
- Rothenbacher D, Capkun G, Uenal H, Tumani H, Geissbühler Y, Tilson H. New opportunities of real-world data from clinical routine settings in life-cycle management of drugs: example of an integrative approach in multiple sclerosis. Curr Med Res Opin. 2015;31:953–65.View ArticlePubMedGoogle Scholar
- Garnock-Jones KP. Alemtuzumab: a review of its use in patients with relapsing multiple sclerosis. Drugs. 2014;74:489–504.View ArticlePubMedGoogle Scholar
- Gelfand JM, Cotter J, Klingman J, Huang EJ, Cree BAC. Massive CNS monocytic infiltration at autopsy in an alemtuzumab-treated patient with NMO. Neurology: Neuroimmunology & Neuroinflammation. 2014;1:e34–4.Google Scholar
- Azzopardi L, Cox AL, McCarthy CL, Jones JL, Coles AJ. Alemtuzumab use in neuromyelitis optica spectrum disorders: a brief case series. J Neurol. 2015;263:1–5.Google Scholar
- Fujihara K, Nakashima I. Secondary progression and innate immunity in NMO: A possible link to alemtuzumab therapy? Neurology: Neuroimmunology & Neuroinflammation. 2014;1:e38–8.Google Scholar
- Nali LHDS, Moraes L, Fink MCD, Callegaro D, Romano CM, Oliveira ACP D. Natalizumab treatment for multiple sclerosis: updates and considerations for safer treatment in JCV positive patients. Arq Neuropsiquiatr. 2014;0:0.Google Scholar
- Tuohy O, Costelloe L, Bjornson I, Harding K, Robertson N, May K, et al. Alemtuzumab treatment of multiple sclerosis: long-term safety and efficacy. J Neurol Neurosurg Psychiatr. 2014;0:1–8.Google Scholar
- Thomas K, Ziemssen T. Management of fingolimod in clinical practice. Clin Neurol Neurosurg. 2013;115(1):S60–4.View ArticlePubMedGoogle Scholar
- Berger JR, Centonze D, Comi G, Confavreux C, Cutter G, Giovannoni G, et al. Considerations on discontinuing natalizumab for the treatment of multiple sclerosis. Ann Neurol. 2010;68:409–11.View ArticlePubMedGoogle Scholar
- Hauser SL, Johnston SC. Balancing risk and reward: the question of natalizumab. Ann Neurol. 2009;66:A7–8.Google Scholar
- Ziemssen T, Hillert J, Butzkueven H. The importance of collecting structured clinical information on multiple sclerosis. BMC Med. 2016;14:81.Google Scholar
- Diel R, Hauer B, Loddenkemper R, Manger B, Krüger K. Recommendations for tuberculosis screening before initiation of TNF-alpha-inhibitor treatment in rheumatic diseases. Pneumologie. 2009;63:329–34.View ArticlePubMedGoogle Scholar
- Caon C, Namey M, Meyer C, Mayer L, Oyuela P, Margolin DH, et al. Prevention and Management of Infusion-Associated Reactions in the Comparison of Alemtuzumab and Rebif ®Efficacy in Multiple Sclerosis (CARE-MS) Program. Int J MS Care. 2015;17:191–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Moreau T, Coles A, Wing M, Isaacs J, Hale G, Waldmann H, et al. Transient increase in symptoms associated with cytokine release in patients with multiple sclerosis. Brain. 1996;119(Pt 1):225–37.View ArticlePubMedGoogle Scholar
- Wing MG, Moreau T, Greenwood J, Smith RM, Hale G, Isaacs J, et al. Mechanism of first-dose cytokine-release syndrome by CAMPATH 1-H: involvement of CD16 (FcgammaRIII) and CD11a/CD18 (LFA-1) on NK cells. J Clin Invest American Society for Clinical Investigation. 1996;98:2819–26.View ArticleGoogle Scholar
- Rebello P, Hale G. Pharmacokinetics of CAMPATH-1H: assay development and validation. J Immunol Methods. 2002;260:285–302.View ArticlePubMedGoogle Scholar
- Klotz L, Berthele A, Bruck W, Chan A, Flachenecker P, Gold R, et al. Monitoring of blood parameters under course-modified MS therapy: Substance-specific relevance and current recommendations for action. Nervenarzt: Springer Berlin Heidelberg; 2016. p. 1–14.Google Scholar
- Tsourdi E, Gruber M, Rauner M, Blankenburg J, Ziemssen T, Hofbauer LC. Graves’ disease after treatment with Alemtuzumab for multiple sclerosis. Hormones (Athens). 2015;14(1):148–53.Google Scholar
- Williams KM, Dietzen D, Hassoun AA, Fennoy I, Bhatia M. Autoimmune thyroid disease following alemtuzumab therapy and hematopoietic cell transplantation in pediatric patients with sickle cell disease. Pediatr Blood Cancer. 2014;61(12):2307–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Aranha AA, Amer S, Reda ES, Broadley SA, Davoren PM. Autoimmune Thyroid Disease in the Use of Alemtuzumab for Multiple Sclerosis: A Review. Endocr Pract. 2013;11:1–25.Google Scholar
- Cuker A, Coles AJ, Sullivan H, Fox E, Goldberg M, Oyuela P, et al. A distinctive form of immune thrombocytopenia in a phase 2 study of alemtuzumab for the treatment of relapsing-remitting multiple sclerosis. Blood. 2011;118:6299–305.View ArticlePubMedGoogle Scholar
- Jones JL, Coles AJ. Mode of action and clinical studies with alemtuzumab. Exp Neurol. 2014;262:37–43.View ArticlePubMedGoogle Scholar
- Daniels GH, Vladic A, Brinar V, Zavalishin I, Valente W, Oyuela P, et al. Alemtuzumab-Related Thyroid Dysfunction in a Phase 2 Trial of Patients With Relapsing-Remitting Multiple Sclerosis. J Clin Endocrinol Metab. 2014;99:80–9.View ArticlePubMedGoogle Scholar
- Haase R, Schultheiss T, Kempcke R, Thomas K, Ziemssen T. Use and acceptance of electronic communication by patients with multiple sclerosis: a multicenter questionnaire study. J Med Internet Res. 2012;14, e135.View ArticlePubMedPubMed CentralGoogle Scholar
- Ziemssen T, Engelmann U, Jahn S, Leptich A, Kern R, Hassoun L, et al. Rationale, Design, and Methods of a Non-interventional Study to Establish Safety, Effectiveness, Quality of Life, Cognition, Health-Related and Work Capacity Data on Alemtuzumab in Multiple Sclerosis Patients in Germany (TREAT-MS). BMC Neurol. 2016. in press.Google Scholar
- Ziemssen T, Kern R, Cornelissen C. The PANGAEA study design–a prospective, multicenter, non-interventional, long-term study on fingolimod for the treatment of multiple sclerosis in daily practice. BMC Neurol BMC Neurology. 2015;18:1–8.Google Scholar
- Ziemssen T, Kempcke R, Eulitz M, Großmann L, Suhrbier A, Thomas K, et al. Multiple sclerosis documentation system (MSDS): moving from documentation to management of MS patients. J Neural Transm. 2013;120 Suppl 1:61–6.View ArticleGoogle Scholar
- Rieckmann P, Boyko A, Centonze D, Elovaara I, Giovannoni G, Havrdova E, et al. Achieving patient engagement in multiple sclerosis_ A perspective from the multiple sclerosis in the 21st Century Steering Group. Multiple Sclerosis and Related Disorders. 2015;4:202–18.View ArticlePubMedGoogle Scholar
- Schultheiß T, Kempcke R, Kratzsch F, Eulitz M, Pette M, Reichmann H, et al. Multiple sclerosis management system 3D: Moving from documentation towards management of patients. Nervenarzt: Springer Berlin Heidelberg; 2011.Google Scholar
- Rau D, Lang M, Harth A, Naumann M, Weber F, Tumani H, et al. Listeria Meningitis Complicating Alemtuzumab Treatment in Multiple Sclerosis--Report of Two Cases. IJMS. 2015;16:14669–76.View ArticlePubMedPubMed CentralGoogle Scholar
- Thomas K, Dietze K, Wehner R, Metz I, Tumani H, Schultheiss T, et al. Accumulation and therapeutic modulation of 6-sulfo LacNAc (+) dendritic cells in multiple sclerosis. Neurology: Neuroimmunology & Neuroinflammation. 2014;1:e33–3.Google Scholar
- Morrison VA. Immunosuppression associated with novel chemotherapy agents and monoclonal antibodies. Clin Infect Dis. 2014;59(5):S360–4.View ArticlePubMedGoogle Scholar
- Coles AJ. Alemtuzumab therapy for multiple sclerosis. Neurotherapeutics. 2013;10:29–33.View ArticlePubMedGoogle Scholar
- Safdar N, Smith J, Knasinski V, Sherkow C, Herrforth C, Knechtle S, et al. Infections after the use of alemtuzumab in solid organ transplant recipients: a comparative study. Diagn Microbiol Infect Dis. 2010;66:7–15.View ArticlePubMedGoogle Scholar
- Ibitoye R, Wilkins A. Thyroid papillary carcinoma after alemtuzumab therapy for MS. J Neurol. 2014;261(9):1828–9.View ArticlePubMedGoogle Scholar
- Brown W, Coles A. Alemtuzumab: evidence for its potential in relapsing–remitting multiple sclerosis. Drug Des Devel Ther. 2013;7:131.View ArticlePubMedPubMed CentralGoogle Scholar
- Coles AJ, Fox E, Vladic A, Gazda SK, Brinar V, Selmaj KW, et al. Alemtuzumab more effective than interferon-1a at 5-year follow-up of CAMMS223 Clinical Trial. Neurology. 2012;78:1069–78.View ArticlePubMedGoogle Scholar
- McCarthy CL, Tuohy O, Compston DAS, Kumararatne DS, Coles AJ, Jones JL. Immune competence after alemtuzumab treatment of multiple sclerosis. Neurology. 2013;81:872–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang X, Tao Y, Chopra M, Ahn M, Marcus KL, Choudhary N, et al. Differential Reconstitution of T Cell Subsets following Immunodepleting Treatment with Alemtuzumab (Anti-CD52 Monoclonal Antibody) in Patients with Relapsing-Remitting Multiple Sclerosis. J Immunol. 2013;191:5867–74.View ArticlePubMedGoogle Scholar
- Robertson NP, Scolding NJ. Immune reconstitution and treatment response in multiple sclerosis following alemtuzumab. Neurology. 2014;82:2150–1.View ArticlePubMedGoogle Scholar
- Tchao NK, Turka LA. Lymphodepletion and homeostatic proliferation: implications for transplantation. Am J Transplant. 2012;12:1079–90.View ArticlePubMedGoogle Scholar
- Vukusic S, Marignier R. Multiple sclerosis and pregnancy in the ‘treatment era’. Nat Rev Neurol. 2015;11:280–9.View ArticlePubMedGoogle Scholar